Candidatus Defluviifilum

Genus nameCandidatus Defluviifilum
SourceMiDAS
Alternative namesEikelboom type 0803, P2CN44 (MiDAS 1.20), Isolate ET1

Taxonomy

KingdomBacteria
PhylumChloroflexi

16S gene copy number-
GenomesNo

 In situOther
Filamentous23
Hydrophobic cell surface2


Industrial type 0803 detected with probe T0803ind-642 (red) and EUBMIX (green). Bar indicates 10 microns. - Source:2

Aerobic heterotroph
Fermentation
Sugars

POSNEGVariableNot assessed

Description

In situ, the organisms are observed to be filamentous with a type 0803 morphotype, which has long been associated with bulking episodes. They have a hydrophobic cell wall supporting a possible role in foaming 2. Suggested to prefer mono- and poly-saccharides. Heterotrophic with able to assimilate glucose and N-acetylglucosamine (low uptake). Glucose is assimilated under anaerobic conditions - with or without addition of nitrite/nitrate although slightly higher with the addition of nitrate. High exoenzymic activity including esterase, glucuronidase with some filaments also having lipase, chitinase and phosphatase activity 2. Conversely, the sole isolate ET1 has a morphology more similar to Halicomenobacter and can not utilise complex carbon sources or ferment. It can utilise galactose, amino acids and acetate aerobically, but not glucose 3.

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Distribution plant/process configuration

Probe T0803-0654 targets members in municiple WWTPs and T0803-0642 is recommended to target those present in industrial WWTPs. Relatively less abundant during the summer in Denmark 1.

FISH probes

T0803-0654 (activated sludge sub-group); T0803ind-0642 (activated sludge sub-group)2

Metabolism

 In situOther
Autotroph/Mixotroph3
AOB3
NOB3
Anammox
Aerobic Heterotroph23
PAO2
GAO2
Nitrite Reduction2
Sulphate Reduction3
Fermentation23
Acetogen
Methanogen
Fatty Acids2
Sugars23
Proteins/Amino Acids23

Abundance Information

 10 % percentileMedian90 % percentile
Influent000.1
Activated Sludge0.412.3
Digester-Mesophilic0.10.51.8
Digester-Thermophilic0.10.20.6

Predominant InActivated sludge

References

[1] Mielczarek, Kragelund, Eriksen, Nielsen - Population dynamics of filamentous bacteria in Danish wastewater treatment plants with nutrient removal. - Water Res. 46(12): 3781-95. doi:10.1016/j.watres.2012.04.009

[2] Kragelund, Thomsen, Mielczarek, Nielsen - Eikelboom's morphotype 0803 in activated sludge belongs to the genus Caldilinea in the phylum Chloroflexi. - FEMS Microbiol. Ecol. 76(3): 451-62. doi:10.1111/j.1574-6941.2011.01065.x

[3] Yoon, Park, Kim, Jeon, Chae, Rhee, et al. - Isolation, characterization, and abundance of filamentous members of Caldilineae in activated sludge. - J. Microbiol. 48(3): 275-83. doi:10.1007/s12275-010-9366-8

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The MiDAS taxonomy is a manual curation of the Silva database and is redistributed for non-commercial use only.

 

Citing MiDAS: McIlroy S.J., Saunders A.M., Albertsen M., Nierychlo M., McIlroy B., Hansen A.A., Karst S.M., Nielsen J.L., Nielsen P.H. (2015) MiDAS: the field guide to the microbes of activated sludge. Database, Vol. 2015

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