Industrial type 0803 detected with probe T0803ind-642 (red) and EUBMIX (green). Bar indicates 10 microns. - Source:2Aerobic heterotroph
In situ, the organisms are observed to be filamentous with a type 0803 morphotype, which has long been associated with bulking episodes. They have a hydrophobic cell wall supporting a possible role in foaming 2. Suggested to prefer mono- and poly-saccharides. Heterotrophic with able to assimilate glucose and N-acetylglucosamine (low uptake). Glucose is assimilated under anaerobic conditions - with or without addition of nitrite/nitrate although slightly higher with the addition of nitrate. High exoenzymic activity including esterase, glucuronidase with some filaments also having lipase, chitinase and phosphatase activity 2. Polyphosphate granules are occasionally observed2. Conversely, the sole isolate ET1 has a morphology more similar to Halicomenobacter and can not utilise complex carbon sources or ferment. It can utilise galactose, amino acids and acetate aerobically, but not glucose 3.
Probe T0803-0654 targets members in municiple WWTPs and T0803-0642 is recommended to target those present in industrial WWTPs. Relatively less abundant during the summer in Denmark 1.
T0803-0654 (activated sludge sub-group); T0803ind-0642 (activated sludge sub-group)2
 Mielczarek, Kragelund, Eriksen, Nielsen (2012): Population dynamics of filamentous bacteria in Danish wastewater treatment plants with nutrient removal. Water Res. 46(12): 3781-95. doi:10.1016/j.watres.2012.04.009
 Kragelund, Thomsen, Mielczarek, Nielsen (2011): Eikelboom's morphotype 0803 in activated sludge belongs to the genus Caldilinea in the phylum Chloroflexi. FEMS Microbiol. Ecol. 76(3): 451-62. doi:10.1111/j.1574-6941.2011.01065.x
 Yoon, Park, Kim, Jeon, Chae, Rhee, et al. (2010): Isolation, characterization, and abundance of filamentous members of Caldilineae in activated sludge. J. Microbiol. 48(3): 275-83. doi:10.1007/s12275-010-9366-8
 Kindaichi, Nierychlo, Kragelund, Nielsen, Nielsen (2013): High and stable substrate specificities of microorganisms in enhanced biological phosphorus removal plants. Environ. Microbiol. 15(6): 1821-31. doi:10.1111/1462-2920.12074