Confocal laser-scanning microscopy image of FISH probed WWTP biomass. Magenta cells are those responding to both CFXmix (CY5) and CFX223 (CY3) FISH probes for B45. Blue cells are those responding to the CFXmix probes alone. Blue and magenta cells lack EUBmix FISH target sites; green cells are those responding only to the EUBmix (FLUOS) probes; light blue cells are those responding to EUBmix plus CFXmix probes together. - Source:2Chemoautotroph/Mixotroph
Filamentous organisms with an Eikelboom type 0092 morphotype, often located inside flocs. They are potentially important for floc structure 2 3 and have long been associated with bulking episodes, although a direct link between their proliferation and bulking episodes has not been established. A metabolic model was constructed for this organism, based on annotation of its genome, showing its ability to generate energy by respiration, utilizing oxygen, nitrite or nitrous oxide as electron acceptors, or by fermentation of sugars. The ability of B45 members to ferment sugars under anaerobic conditions was validated in situ 1.
Anecdotal evidence suggest they are relatively more abundant in EBPR plants 2.
CFX197 + CFX223 2
 McIlroy, Karst, Nierychlo, Dueholm, Albertsen, Kirkegaard, et al. (2016): Genomic and in situ investigations of the novel uncultured Chloroflexi associated with 0092 morphotype filamentous bulking in activated sludge. ISME J 10(9): 2223-34. doi:10.1038/ismej.2016.14
 Speirs, Nittami, McIlroy, Schroeder, Seviour (2009): Filamentous bacterium Eikelboom type 0092 in activated sludge plants in Australia is a member of the phylum Chloroflexi. Appl. Environ. Microbiol. 75(8): 2446-52. doi:10.1128/AEM.02310-08
 Mielczarek, Kragelund, Eriksen, Nielsen (2012): Population dynamics of filamentous bacteria in Danish wastewater treatment plants with nutrient removal. Water Res. 46(12): 3781-95. doi:10.1016/j.watres.2012.04.009