FISH image of Ca. Villogracilis (red) in full-scale activated sludge. Other cells appear blue. Bar= 20 microns. - Source:1Aerobic heterotroph
Thin filaments of 0.3 – 0.4 µm wide and 12 – 50 µm long. Often observed in bundles and almost exclusively located within the flocs1.
CFX763A and CFU763B target subgroup A and B, respectively. They collectively cover >60% of the genus sequences in the MiDAS database 1. The helper probes CFX763ABH1A and CFX763ABH1B are recommended to give optimal fluorescence signal 1.
 Nierychlo, Milobedzka, Petriglieri, McIlroy, Nielsen, McIlroy, et al. (2019): The morphology and metabolic potential of the Chloroflexi in full-scale activated sludge wastewater treatment plants. FEMS Microbiol. Ecol. 95 (2): . doi:10.1093/femsec/fiy228