Confocal laser-scanning microscopy image of FISH probed AD biomass. Red filaments are hybridized with CFX-A6-mix probe set, while green filaments are positive to hybridization with MX825mix. The image shows co-localization of A6 and Methanosaeta sp. Scale bar = 10 microns. - Source:1Fermentation
Filamentous organisms distributed throughout the flocs and often detected co-located with methanogenes, suggesting a possible synergistic relationship. Genomic reconstruction revealed a strictly anaerobic metabolism and fermentative physiology. The ability of A6 members has not been validated in situ 1.
CFX-A6-450 and CFX-A6-1278. The two FISH probes can be used together to give a higher coverage of the group.
 McIlroy, Kirkegaard, Dueholm, Fernando, Karst, Albertsen, et al. (2017): Culture-Independent Analyses Reveal Novel Anaerolineaceae as Abundant Primary Fermenters in Anaerobic Digesters Treating Waste Activated Sludge. Front Microbiol 8 (): 1134. doi:10.3389/fmicb.2017.01134