FISH image of activated sludge after color combination showing Bacteria hybridized with EUBmix (Cy5 labeled, set as green) as cells labeled with different colors. Epiflora hybridized with Bac111 (FLUOS labeled, set as blue) as cyan-labeled cells (mixture of green and blue, indicated by arrows b), and epiflora hybridized with EpiMix (Cy3 labeled, set as red) as purple-labeled cells (mixture of blue, red, and green, indicated by arrows a). Scale bar represents 10 microns. - Source:1Aerobic heterotroph
"Ca. Epiflobacter" grow as rods attached to filamentous organisms. They are likely involved in protein degradation with surface-linked protease activity and aerobic uptake of amino acids shown in situ 1.
3 FISH probe defined sub-groups in activated sludge 1
Cluster 1 = Epi993A; Cluster 2 = Epi993B; Cluster 3 = Epi1004; All = Epi741 1
 Xia, Kong, Thomsen, Halkjaer Nielsen (2008): Identification and ecophysiological characterization of epiphytic protein-hydrolyzing saprospiraceae ("Candidatus Epiflobacter" spp.) in activated sludge. Appl. Environ. Microbiol. 74 (7): 2229-38. doi:10.1128/AEM.02502-07