MethanothermobacterDid we forget anything? Let us know

Genus nameMethanothermobacter
SourcePublished
Alternative namesMethanobacterium
NCBI taxonomy ID145260

Taxonomy

KingdomArchaea

16S gene copy number2
GenomesYes23456

 In situOther
Filamentous78912
Hydrophobic cell surface


FISH image of M. thermautotrophicus DSM1053T. Bars, 10 microns. - Source:7

Autotroph/Mixotroph
Methanogen
Fatty acids

POSNEGVariableNot assessed

Description

Methanothermobacter are members of the domain Archaea, renowned for their methanogenic ability. They are hydrogenotrophic methanogens, which convert H2 + CO2 to methane 8. Growth occurs under strictly anaerobic conditions, whereby acetate may be assimilated or required by some species 8 9. Autotrophic growth is also possible by members of the genus 8. Methanothermobacter form natural syntrophic associations with bacteria including the genera Coprothermobacter 1 and Pelatomaculum 10 as examples. In pure culture cells appear as curved or crooked slender rods, which may occur singly, in pairs or as moderately long chains to filaments 8.

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FISH probes

MB1174 and MB310 specific for the family Methanobacteriaceae 11. Pseudomurein endoisopeptidase (Pei) treatment may be recommended prior to FISH studies to improve probe hybridization 7.

Metabolism

 In situOther
Autotroph/Mixotroph8
AOB
NOB
Anammox
Aerobic Heterotroph8
PAO
GAO
Nitrite Reduction
Sulphate Reduction
Fermentation
Acetogen
Methanogen8913
Fatty Acids89
Sugars
Proteins/Amino Acids

Abundance Information

 10 % percentileMedian90 % percentile
Influent000
Activated Sludge000
Digester-Mesophilic000.1
Digester-Thermophilic59.770.383.9

Predominant InAD - Thermophilic

References

[1] Sasaki, Morita, Sasaki, Nagaoka, Matsumoto, Ohmura, et al. - Syntrophic degradation of proteinaceous materials by the thermophilic strains Coprothermobacter proteolyticus and Methanothermobacter thermautotrophicus. - J. Biosci. Bioeng. 112(5): 469-72. doi:10.1016/j.jbiosc.2011.07.003

[2] - NCBI genome database, NCBI id 145260 -

[3] Kosaka, Toh, Fujiyama, Sakaki, Watanabe, Meng, et al. - Physiological and genetic basis for self-aggregation of a thermophilic hydrogenotrophic methanogen, Methanothermobacter strain CaT2. - Environ Microbiol Rep 6(3): 268-77. doi:10.1111/1758-2229.12128

[4] Kosaka, Toh, Toyoda - Complete Genome Sequence of a Thermophilic Hydrogenotrophic Methanogen, Methanothermobacter sp. Strain CaT2. - Genome Announc 1(4): . doi:10.1128/genomeA.00672-13

[5] Liesegang, Kaster, Wiezer, Goenrich, Wollherr, Seedorf, et al. - Complete genome sequence of Methanothermobacter marburgensis, a methanoarchaeon model organism. - J. Bacteriol. 192(21): 5850-1. doi:10.1128/JB.00844-10

[6] Smith, Doucette-Stamm, Deloughery, Lee, Dubois, Aldredge, et al. - Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics. - J. Bacteriol. 179(22): 7135-55.

[7] Nakamura, Terada, Sekiguchi, Shinzato, Meng, Enoki, et al. - Application of pseudomurein endoisopeptidase to fluorescence in situ hybridization of methanogens within the family Methanobacteriaceae. - Appl. Environ. Microbiol. 72(11): 6907-13. doi:10.1128/AEM.01499-06

[8] Wasserfallen, Nölling, Pfister, Reeve, Conway de Macario - Phylogenetic analysis of 18 thermophilic Methanobacterium isolates supports the proposals to create a new genus, Methanothermobacter gen. nov., and to reclassify several isolates in three species, Methanothermobacter thermautotrophicus comb. nov., Methanothermobacter wolfeii comb. nov., and Methanothermobacter marburgensis sp. nov. - Int. J. Syst. Evol. Microbiol. 50 Pt 1(): 43-53. doi:10.1099/00207713-50-1-43

[9] Cheng, Dai, Li, Zhang, Lu - Isolation and characterization of Methanothermobacter crinale sp. nov., a novel hydrogenotrophic methanogen from the Shengli oil field. - Appl. Environ. Microbiol. 77(15): 5212-9. doi:10.1128/AEM.00210-11

[10] Ishii, Kosaka, Hori, Hotta, Watanabe - Coaggregation facilitates interspecies hydrogen transfer between Pelotomaculum thermopropionicum and Methanothermobacter thermautotrophicus. - Appl. Environ. Microbiol. 71(12): 7838-45. doi:10.1128/AEM.71.12.7838-7845.2005

[11] Raskin, Stromley, Rittmann, Stahl - Group-specific 16S rRNA hybridization probes to describe natural communities of methanogens. - Appl. Environ. Microbiol. 60(4): 1232-40.

[12] Nakamura, Takahashi, Mori, Tamaki, Mochimaru, Nakamura, et al. - Methanothermobacter tenebrarum sp. nov., a hydrogenotrophic, thermophilic methanogen isolated from gas-associated formation water of a natural gas field. - Int. J. Syst. Evol. Microbiol. 63(Pt 2): 715-22. doi:10.1099/ijs.0.041681-0

[13] Kawaguchi, Sakuma, Nakata, Kobayashi, Endo, Sato, et al. - Methane production by Methanothermobacter thermautotrophicus to recover energy from carbon dioxide sequestered in geological reservoirs. - J. Biosci. Bioeng. 110(1): 106-8. doi:10.1016/j.jbiosc.2010.01.008

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